TB-500 Dosage in Animal Tests | TB-500 Safe
Researchers used many amounts and shot sites. Most tests involved rodents or horses.
One human study tested the full Thymosin Beta-4 protein [16]. It didn't test TB-500.
Here’s the short version. Nobody has found a safe human TB-500 amount.
The figures below only report what each test used. Keep the animal and injury beside every amount.
A rat amount can't be moved into your own care. Neither can a horse shot schedule.
TB-500 has no tested human dose in these papers. This list isn't a plan for taking it.
What TB-500 shot schedules came from horse reports
Horse reports mention 2–5 milligrams of TB-500. Shots were given 1–2 times each week for 4–6 weeks [18].
Some sellers call that a loading plan. They mean a starting run of shots.
The horse reports are also where the 4–6 week span came from. No human study tested that schedule.
Researchers didn't prove it was safe for horses or people. They only reported the horse use.
If these figures appear in a human plan, remember their source. They came from horse reports, not a human safety study.
What amount each animal test used
These are the amounts reported for Thymosin Beta-4 and TB-500. They aren't a guide for human use.
- 5.37 milligrams for each kilogram a rat weighed: rats weighed at least 250 grams. They had 43% less heart damage at 28 days [6].
- 6 or 30 milligrams per kilogram: rats with brain injury moved and learned better at 30 milligrams per kilogram [7].
- 2–18 milligrams per kilogram: a rat stroke test calculated 3.75 milligrams per kilogram as best at day 56 [8].
- 1 microgram at the wound: one microgram means one part in a million of a gram. The cut rat knee ligament was stronger by 4 weeks [9].
- 150 micrograms twice weekly for 6 months: mouse muscle grew more fibers. The mice didn't grip better [11].
- 12 milligrams per kilogram each day for 4 weeks: sick mice had fewer swelling cells. Immune cells that clear damage became more common [22].
- As little as 10 trillionths of a gram: dish-grown skin cells moved. That amount wasn't injected into an animal [3].
- 0.05–25 micrograms per kilogram once; 0.5–5.0 micrograms per kilogram for a 10-day test: Thymosin Beta-4 went to 54 healthy people, not TB-500 [16].
The list mixes animals, dishes, and full-protein work. It also mixes injuries and shot sites.
An amount from one test can't set another test's amount. It can't set a human amount either.
How long TB-500 pieces stayed in blood
Researchers haven't measured a TB-500 shot's half-life in people. A drug's half-life is the time its blood level takes to fall by half.
Thymosin Beta-4 in people: In 54 healthy people, that level took 0.5–2.08 hours to fall by half [16]. The highest level came at 3–15 minutes.
Researchers gave the full protein through a vein. They didn't use TB-500.
TB-500 in rats: A 2024 test found two broken-down parts after a shot [19]. One was highest at 0–6 hours.
The other could still be found at 72 hours. The first part helped repair cells close a gap in a dish.
None of the tested parts killed those cells. Rat blood held them for 6–72 hours.
The span changed with the part being measured. It doesn't set how often TB-500 shots could be given.
Nobody knows TB-500 timing in people. No published TB-500 blood test has answered it.
What TB-500 tests say about time between shots
Most animal tests gave shots weekly or twice weekly [19]. Daily TB-500 use was rare in the papers.
No test found safe spacing for people. More shots may bring risks nobody has measured.
Where animal test shots went into the body
Most rodent tests put shots into the belly area. No approved TB-500 care exists for people.
Horse and some rodent reports used shots beneath skin or in muscle. No human study compared those two places.
The human Thymosin Beta-4 study used a vein [16]. A vein sends a drug into blood at once.
The shot site can change how fast a drug acts. These tests don't name a safe human shot site.
When TB-500 animal tests looked for change
Rat wound tests checked at 4–7 days [3]. Ligament work checked at 4 weeks [9].
Rat blood held broken-down pieces from 0 to 72 hours [19]. Horse reports used 4–6 week shot plans.
Those are check dates, not known healing times. No human test found when TB-500 starts or stops acting.
How long animal tests kept giving TB-500
Most animal tests ran 4–8 weeks. Researchers chose those dates to check repair.
One 6-month mouse test followed sick muscle [11]. No published test went past 6 months.
None tried another course after a break. Those animal dates can't set your schedule.
An end date doesn't mean healing was done. It only tells you when researchers looked.
What daily and weekly animal tests found
Most animal work used weekly or twice-weekly shots [11][18][22]. Daily use appeared in one mouse liver test.
That test used 12 milligrams per kilogram each day for 4 weeks [22]. Researchers chose it for one mouse illness.
It doesn't show daily human use is safe or needed. No published test has checked daily shots in people.
A daily plan didn't come from a human trial. The mouse test can't set your calendar.
What lab tests found inside TB-500 vials
A 2023 lab study checked two related products sold online [17]. Their contents didn't always match the labels.
A vial may also hold germs, metals, or the wrong peptide. Those risks are separate from the drug's effects.
FDA once put TB-500 in Category 2. Safety concerns then barred pharmacy-made use in people.
On April 22, 2026, FDA's page showed that a review request had been withdrawn. The request sought review for pharmacy-made use; FDA didn't name who filed it.
Ending the review wasn't approval or proof of safety. FDA still noted concerns about unwanted contents.
A label can't prove one vial is clean. Only a proper lab test can check that.